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plasma pai 1  (Innovative Research Inc)


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    Innovative Research Inc plasma pai 1
    Plasma Pai 1, supplied by Innovative Research Inc, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+pai+1/Mouse+PAI-1+Genetically+Deficient+Plasma%2CMouse+PAI-1+genetically+deficient+plasma%2C+sodium+citrate/pm41912791-421-0-9
    Average 93 stars, based on 9 article reviews
    plasma pai 1 - by Bioz Stars, 2026-09
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    Related Articles

    Fluorescence:

    Article Title: Plasminogen activator inhibitor-1 inhibitors and methods of use thereof to modulate lipid metabolism
    Article Snippet: The plate was vacuumed and washed 3× with wash buffer and 100 μl of sheath fluid (Luminex) was added to each well, shaken for 5 minutes in dark at room temperature and read on a Luminex100 (median setting, 50 μl sample size, 100 events/bead). .. The mean fluorescence intensity (MFI) of the unknown sample was then converted into a pg/mL value based on a standard curve of active mouse PAI-1 in PAI-1 depleted mouse plasma (Molecular Innovations) using a five-parameter regression formula (Masterplex 2.0, Miraibio). .. Data were analyzed and IC50 values calculated using GraFit 5 (Erithacus Software Limited).

    Clinical Proteomics:

    Article Title: Plasminogen activator inhibitor-1 inhibitors and methods of use thereof to modulate lipid metabolism
    Article Snippet: The plate was vacuumed and washed 3× with wash buffer and 100 μl of sheath fluid (Luminex) was added to each well, shaken for 5 minutes in dark at room temperature and read on a Luminex100 (median setting, 50 μl sample size, 100 events/bead). .. The mean fluorescence intensity (MFI) of the unknown sample was then converted into a pg/mL value based on a standard curve of active mouse PAI-1 in PAI-1 depleted mouse plasma (Molecular Innovations) using a five-parameter regression formula (Masterplex 2.0, Miraibio). .. Data were analyzed and IC50 values calculated using GraFit 5 (Erithacus Software Limited).

    Article Title: Plasminogen activator inhibitor 1 and venous thrombosis in pancreatic cancer
    Article Snippet: Commercial ELISAs were used to measure levels of active mouse PAI-1 (#MPAIKT; Molecular Innovations) and active human PAI-1 (#HPAIKT; Molecular Innovations) in plasma from mice with or without tumors. .. Commercial ELISAs were used to measure levels of active mouse PAI-1 (#MPAIKT; Molecular Innovations) and active human PAI-1 (#HPAIKT; Molecular Innovations) in plasma from mice with or without tumors. .. Plasma levels of thrombin-antithrombin (TAT) complexes (#OWMG15; Siemens, Munich, Germany) in mice were measured using a commercial assay.

    other:

    Article Title: Attenuation of Ventilation-Enhanced Epithelial–Mesenchymal Transition through the Phosphoinositide 3-Kinase-γ in a Murine Bleomycin-Induced Acute Lung Injury Model
    Article Snippet: PAI-1 (0.02 ng/mL) and VEGF (1.8 pg/mL) were detected in serum and bronchoalveolar lavage fluid using immunoassay kits containing primary polyclonal anti-mouse antibodies that were cross-reactive with rat and mouse PAI-1 and VEGF (VEGF: Biosource International, Camarillo, CA, USA; PAI-1: Molecular Innovations Inc., Plymouth Meeting, PA, USA).

    Article Title: Ethyl pyruvate attenuates ventilation‐induced diaphragm dysfunction through high‐mobility group box‐1 in a murine endotoxaemia model
    Article Snippet: PAI‐1, with a lower detection limit of 0.02 ng/mL, and HMGB1 (1 ng/mL) were detected in bronchoalveolar lavage (BAL) fluid using immunoassay kits containing primary polyclonal anti‐mouse antibodies that were cross‐reactive with rat and mouse PAI‐1 and HMGB1 (PAI‐1: Molecular Innovations, Inc, Southfield, MI; HMGB1: Shino‐Test corporation, Kanagawa, Japan).

    Mutagenesis:

    Article Title: Antibodies to plasminogen activator inhibitor-1 (PAI-1) and uses thereof
    Article Snippet: To finalize reaction 200 ul tPA substrate S2288 (Chromogenix) (1.25 mM) was added to the mixture. .. Ortholog PAI-1 was obtained from Molecular Innovations: mouse PAI-1 (wild type active fraction; cat #MPAI); rat PAI-1 (wild type active fraction; cat #RPAI); and rabbit PAI-1 (stable mutant; cat #RbPAI-I91L) cyno PAI-1 (active cyno PAI-1) was produced in-house in E. coli. .. Because of the poor off-rates of the rabbit and rat orthologs in the Biacore screening (data not shown), screening of the antibodies against these orthologs was not performed.

    Article Title: Vitronectin stabilizes intravascular adhesion of neutrophils by coordinating b2 integrin clustering
    Article Snippet: ; R&D Systems; Minneapolis, MI) was used to induce leukocyte recruitment. .. In selected experiments, mouse PAI-1 (CPAI; 50 μg in 100 μl PBS; Molecular Innovations, Novi, MI) and the PAI-1 mutant proteins PAI-QR or PAI-RR (50 μg in 100 μl PBS; Molecular Innovations) were applied i.v. to PAI-1-/- mice. .. Activation of mouse neutrophils was measured upon exposure to mouse VN, PAI-1, uPA, VN-PAI-1, or VN-uPA (Molecular Innovations).

    Produced:

    Article Title: Antibodies to plasminogen activator inhibitor-1 (PAI-1) and uses thereof
    Article Snippet: To finalize reaction 200 ul tPA substrate S2288 (Chromogenix) (1.25 mM) was added to the mixture. .. Ortholog PAI-1 was obtained from Molecular Innovations: mouse PAI-1 (wild type active fraction; cat #MPAI); rat PAI-1 (wild type active fraction; cat #RPAI); and rabbit PAI-1 (stable mutant; cat #RbPAI-I91L) cyno PAI-1 (active cyno PAI-1) was produced in-house in E. coli. .. Because of the poor off-rates of the rabbit and rat orthologs in the Biacore screening (data not shown), screening of the antibodies against these orthologs was not performed.

    Double Immunostaining:

    Article Title: Serpine 1 induces alveolar type II cell senescence through activating p53‐p21‐Rb pathway in fibrotic lung disease
    Article Snippet: .. To reveal PAI‐1, p53, and p21 proteins in mouse lung tissue, double immunostaining was conducted using formalin‐fixed, paraffin‐embedded tissue slides with antibodies to mouse PAI‐1 (Molecular Innovations, Cat No MA‐33H1F7), p53 (Ancell, Cat No 227‐020), or p21 (Santa Cruz, Cat No SC‐6246) and rabbit polyclonal anti‐mouse proSP‐C antibody (Millipore, Cat No AB3786), following the protocol as we have described previously (El‐Deiry et al ., ). ..

    Formalin-fixed Paraffin-Embedded:

    Article Title: Serpine 1 induces alveolar type II cell senescence through activating p53‐p21‐Rb pathway in fibrotic lung disease
    Article Snippet: .. To reveal PAI‐1, p53, and p21 proteins in mouse lung tissue, double immunostaining was conducted using formalin‐fixed, paraffin‐embedded tissue slides with antibodies to mouse PAI‐1 (Molecular Innovations, Cat No MA‐33H1F7), p53 (Ancell, Cat No 227‐020), or p21 (Santa Cruz, Cat No SC‐6246) and rabbit polyclonal anti‐mouse proSP‐C antibody (Millipore, Cat No AB3786), following the protocol as we have described previously (El‐Deiry et al ., ). ..



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    Hemostatic biomarkers in mouse models of acute myeloid leukemia (AML) and acute promyelocytic leukemia (APL). Levels of hemostatic biomarkers in control mice and mice bearing NB4-Luc cells (APL) or HL-60-Luc2 cells (AML) for (A) cell-free DNA (cfDNA), (B) thrombin-antithrombin complexes (TAT), (C) fibrinogen, (D) plasmin-antiplasmin complexes (PAP), (E) D-dimer, and (F) plasminogen activator inhibitor 1 (PAI-1). Control ( n = 7-9), APL mice ( n = 6 at 28 [ n = 2] or 31 [ n = 4] days for cfDNA, TAT and D-dimer, n = 7 at 29 [ n = 1] or 31 [ n = 6] days for fibrinogen, PAP, and PAI-1), and AML mice ( n = 9 at 45 [ n = 2], 48 [ n = 2], or 50 [ n = 5] days for cfDNA, TAT, and D-dimer. n = 6 at 51 days for fibrinogen, PAP, and PAI-1) are shown. (B, C, E, and F) An ordinary one-way anova followed by Tukey’s test was used for TAT, fibrinogen, D-dimer, and PAI-1; (A and D) the Kruskal–Wallis test followed by Dunn’s test was used for cfDNA and PAP. ∗ P < .05; ∗∗ P < .01; ∗∗∗∗ P < .0001.

    Journal: Research and Practice in Thrombosis and Haemostasis

    Article Title: Comparison of changes in blood cells and hemostatic biomarkers in mouse xenograft models of acute myeloid leukemia and acute promyelocytic leukemia

    doi: 10.1016/j.rpth.2025.103319

    Figure Lengend Snippet: Hemostatic biomarkers in mouse models of acute myeloid leukemia (AML) and acute promyelocytic leukemia (APL). Levels of hemostatic biomarkers in control mice and mice bearing NB4-Luc cells (APL) or HL-60-Luc2 cells (AML) for (A) cell-free DNA (cfDNA), (B) thrombin-antithrombin complexes (TAT), (C) fibrinogen, (D) plasmin-antiplasmin complexes (PAP), (E) D-dimer, and (F) plasminogen activator inhibitor 1 (PAI-1). Control ( n = 7-9), APL mice ( n = 6 at 28 [ n = 2] or 31 [ n = 4] days for cfDNA, TAT and D-dimer, n = 7 at 29 [ n = 1] or 31 [ n = 6] days for fibrinogen, PAP, and PAI-1), and AML mice ( n = 9 at 45 [ n = 2], 48 [ n = 2], or 50 [ n = 5] days for cfDNA, TAT, and D-dimer. n = 6 at 51 days for fibrinogen, PAP, and PAI-1) are shown. (B, C, E, and F) An ordinary one-way anova followed by Tukey’s test was used for TAT, fibrinogen, D-dimer, and PAI-1; (A and D) the Kruskal–Wallis test followed by Dunn’s test was used for cfDNA and PAP. ∗ P < .05; ∗∗ P < .01; ∗∗∗∗ P < .0001.

    Article Snippet: Levels of different biomarkers in plasma were measured using commercial enzyme-linked immunosorbent assays: TAT (Siemens, cat number OWMG15), fibrinogen (Immunology Consultation Laboratory Inc, cat number E-90FIB), PAP (MyBioSource, cat number MBS2512896), D-dimer (Diagnostica Stago, cat number 00947), and plasminogen activator inhibitor 1 (PAI-1; Molecular Innovations, cat number IMSPAI1KTA). cfDNA was measured using the Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific, cat number P11496).

    Techniques: Control